The six hormones mainly include(Luteinizing hormone LH, prolactin PRL, testosterone testosterone, follicle stimulating hormone FSH, estradiol Estradio, progesterone Progesterone)Mainly detected by ELISA method,Microplate ReaderThe experimental principle for detecting six hormones is as follows:

Application of double antibody sandwich method for determination of human estradiol in specimens(E2)Level. Using purified human estradiol(E2)Antibody coated microplates are used to prepare solid-phase antibodies, and estradiol is sequentially added to the micropores coated with monoclonal antibodies(E2)And then withHRPMarked estradiol(E2)Antibody binding, forming antibodies-antigen-Enzyme labeled antibody complex, washed and substrate addedTMBColor rendering.TMBInHRPUnder the catalysis of enzymes, it is converted into blue, and under the action of acid, it is converted into the final yellow. The depth of color and estradiol in the sample(E2)Positive correlation. Using an enzyme-linked immunosorbent assay (ELISA) reader450nmMeasure absorbance at wavelength（ODCalculate the value of human estradiol in the sample using a standard curve(E2)Concentration.

If some reagent kit manufacturers require dual wavelength detection, they generally use the 450nm wavelength and 630nm wavelength of the ELISA reader for dual wavelength detection.