Voice of VirtueLegionellaLatest release of anti serum

Latest release in October 2025, origin in the United States

R&DScientists in the United StatesCDCwork20 Year, including 1976 During the outbreak of Legionnaires' disease, he helped develop Legionella bacteriaDFAThe test reagents were tested and the first four serum groups of Legionella pneumophila were described. The first monoclonal antibody for detecting Legionella was produced.1984 Starting from the year, we have been producing and supplying various testing kits, specializing inDFAResearch and manufacturing of testing; Including antibodies, antigens, and prepared slides for the identification of Legionella pneumophila serogroups and species. In Legionella Antibody and GoatIgGThe professional knowledge in secondary antibody production is commendable.

Provided by Virtue VoiceLegionellaSpecific AntiserumIt is an in vitro diagnostic reagent containing high titer and high specificity antibodies. Core components of the productforRabbit derived polyclonal antibodySpecially designed for detecting and identifying specificLegionfungusStrain antigen.

Product Core Features

· Product Name: Legionfungusantiserum

· Antibody type: Polyclonal antibodies

· Host/isotypeRabbit/IgG

· specificity: Targeting specific legionsfungusstrain

· form: Liquid

· Coupling stateNon coupling (users can choose their own detection system for coupling according to their needs)

· concentrationTypical quality inspection batch concentration is 4 mg/ml

· Specifications: 1 ml

· Storage buffer solutionPBS, pH 7.6 (without preservatives)

· Storage conditionsRefrigerate at 2-8 ° C and store away from light. Freezing is prohibited (unless otherwise specified).

main purpose

This anti serum reagent is mainly used forIn vitro laboratory researchBy analyzing target antigens in biological, environmental, food, water samples, or research samplesQualitative or quantitative detection, identification, and measurementThe specific application depends on the experimental plan and detection platform chosen by the user (such as enzyme-linked immunosorbent assay, immunofluorescence, etc.).

Important NoticeThis productFor research purposes onlyIt is not directly used for clinical diagnosis of human diseases unless the user has completed sufficient verification and indicated its diagnostic purpose.

1. Rapid diagnosis and monitoring of Legionnaires' disease (main purpose)

→ Rapid preliminary diagnosisWhen there are cases of pneumonia of unknown cause (especially when occurring in groups), CDC or public health laboratories need to quickly identify the pathogen.Direct immunofluorescence antibody detectionResults can be provided within a few hours.

→ workflow：

o Sample TypeProcessing clinical samples, such as respiratory secretions (sputum, bronchoalveolar lavage fluid) or lung tissue slices from patients.

o Testing process: Use this legionfungusAntiserum (as specificity)oneStain the sample according to the 10 step DFA protocol described in document 3, using either anti - or directly fluorescently labeled conjugates.

o Result interpretationObserving under a fluorescence microscope, if there are specific legions present in the samplefungusStrain, its surface antigen will bind with antibodies and emit fluorescence under a microscope, thereby achievingDirect visual inspection and identification.

→ advantageExtremely fast speed is crucial for initiating public health responses and guiding clinical treatment.

2. Detection and typing of Legionella in environmental samples

The important responsibility of CDC is to track the source of infection and prevent the spread of the epidemic. The environmental sources of Legionella bacteria, such as cooling towers, water pipe systems, and water heaters, are the focus of investigation.

→ purposeTesting of environmental samples collected from potential sources of pollution.

→ workflow：

o sample processingConcentrate and preprocess environmental swabs or water samples, possibly for culture amplification, and then prepare them into smears.

o Strain identification: Use thisStrain specific antibodiesDirectly identify the serotype or strain type of environmental isolates through the DFA method. This is forMatch strains in the environment with strains isolated from clinical casesIt is crucial to confirm the transmission chain.

3. As a research method and quality control tool

Even within the CDC, this product is used for a wider range of research and quality assurance activities.

→ Strain confirmation and researchIn laboratory research, it is used to confirm the serotype of bacteria in culture or to conduct pathogen characterization studies.

→ quality controlAs a known positive quality control product, it is used to verify the accuracy and reliability of other detection methods (such as PCR or culture methods).

Technical Principle

The antibodies in the reagent can correspond to the corresponding legion in the samplefungusAntigen occurrencespecific bindingThe type of signal generated after combination (such as agglutination reaction, signal generation, or color change) depends on the detection method used. To ensure accurate results, users need to establish controls for each sample matrix and experimental procedure to distinguish between specific binding and non-specific binding.

Application example: Summary of steps for direct immunofluorescence detection

The document provides information for detecting Legionella bacteriaDirect immunofluorescence methodThe detailed operation process mainly includes the following key steps:

1. Glass slide cleaningClean the glass slide with a specialized cleaning solution, rinse with sterile water, and dry.

2. Antigen fixationApply antigen suspension (such as formalin fixed bacterial suspension) onto a glass slide, air dry, and lightly heat fix.

3. Antibody incubationCover with an appropriate amount of DFA fluorescent labeled conjugate and incubate at room temperature in a wet box for 30 minutes.

4. washGently rinse with PBS buffer and sterile distilled water to remove unbound antibodies.

5. Sealing and ObservationAdd buffer glycerol dropwise, cover with a cover glass, and observe the results under a fluorescence microscope.

Quality Control and Limitations

· Product performance is affected bySample matrix, experimental protocol, and operational techniquesinfluence.

· The test results are only valid when the positive and negative control results meet expectations.

· It is recommended to conduct testing before using the product for new applications or sample typesMethod validation.

Safety and Waste Management

· When using, relevant biosafety regulations and personal protective equipment requirements must be followed.

· Avoid mixing with unverified substances.

· After the experiment, all waste must be disposed of in accordance with institutional and local biosafety regulations.

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Voice of VirtueprovidedLegionfungusAntiserum is a high-quality research tool with strong specificity and wide applications, particularly suitable for precise detection of Legionella in environmental monitoring and scientific research fields.

Order Information

water analysis

ISO 6222 -Total Bacterial Count

ISO 9308 -Escherichia coli and coliforms

ISO 7899 -Enterococcus faecalis

APHAStreptococcus

ISO 11731 -Legionfungus

ISO 16266 -Pseudomonas aeruginosa

ISO 6461- Sulfite reducing Clostridium

Quality control strains

water analysis

ISO 6222 -Total Bacterial Count

ISO 9308 -Escherichia coli and coliforms

ISO 7899 -Enterococcus faecalis

ISO 16266 -Pseudomonas aeruginosa

ISO 6461 -Reduction of sulfite anaerobic sporesfungus

ISO 11731 -Legionfungus

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Voice of Virtueprovidedentire lineMicrobiology and Molecular Biology Productswidely used invarious typesClinical, industrial, and veterinary testingmicroorganism、Drug susceptibility testing laboratory,Public Health Centerlaboratory，CDCDisease Control Center LaboratorywaitNeed highquality inspectionunit.

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