Solid phase extraction device (SPE)It is a sample pretreatment technology device based on liquid-solid chromatography theory, which selectively adsorbs target compounds through solid adsorbents to achieve separation, purification, and concentration of target substances in the sample.

　　Solid phase extraction device (SPE)Technical advantages:

Efficient enrichment: Concentrate trace target substances from large volume samples, such as enriching pesticides in 1L water samples to 1mL, increasing sensitivity by 1000 times.

High selectivity: By optimizing the type of filler, specific separation of target substances in complex matrices can be achieved, such as the separation of drugs and proteins in biological samples.

Easy to operate: The standardized process (activation → sample loading → rinsing → elution) shortens the pre-treatment time, and the processing time for a single sample can be reduced to less than 10 minutes.

Solvent saving: Compared to liquid-liquid extraction, the amount of organic solvents used is reduced by more than 80%, in line with the principles of green chemistry.

Automated compatibility: When used in conjunction with a fully automated solid-phase extraction instrument, high-throughput processing (such as 96 well plate mode) can be achieved, with a daily processing capacity of hundreds of samples.

Operation process:

Activation: Wet the packing with methanol (reverse phase column) or non-polar solvent (normal phase column) to remove impurities and create a solvent environment.

Sample loading: The sample is passed through a small column at a flow rate of 0.5-2mL/min, and the target substance is adsorbed while impurities are discharged with the waste liquid.

Rinsing: Use weak solvents (such as water or buffer) to remove weakly adsorbed interferents and avoid loss of the target substance.

Elution: Use a strong solvent (such as methanol or acidic solution) to elute the target substance into the collection tube, with a typical elution volume of 0.5-2mL.

Concentration: The eluent is concentrated to dryness by nitrogen blowing or vacuum centrifugation, and after reconstitution, chromatographic analysis (such as HPLC, GC-MS) is performed.