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E-mail
luolinlin@jxzdkz.com
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13439158498
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02, 1st to 3rd floors, Building 38, No. 2 Jingyuan North Street, Beijing Economic and Technological Development Zone (Beijing Pilot Free Trade Zone High end Industrial Zone Cluster)
Beijing Jingxiang Environmental Technology Co., Ltd
Sharing of Regular Maintenance Methods for Escherichia coli Online Monitoring System
Date: 2025-09-15Read: 0
The online monitoring instrument for Escherichia coli provides early warning of microbial contamination in water supply networks, water treatment plants, bottled water production lines, and public places by real-time and continuous detection of the concentration of Escherichia coli and total Escherichia coli in water bodies. Its core relies on biosensors, fluorescence detection, and automatic cultivation technology, and long-term operation is easily affected by water quality fluctuations, biofilm adhesion, or reagent depletion. Scientific and systematic regular maintenance of the online monitoring instrument for Escherichia coli is the cornerstone of ensuring its data reliability.
Step 1: Daily cleaning and status inspection (performed by operators)
After each reading or at the end of the shift, check if the indicator light and display screen of the Escherichia coli online monitoring device are functioning properly. Observe the injection pipeline for any bubbles, blockages, or leaks. Rinse the sampling probe and pre filter membrane (if any) with sterile water to prevent algae or particulate matter from adhering. Record the daily detection results and alarm events, and confirm that the data upload is normal.
Step 2: Weekly functional testing and pipeline flushing
Use standard bacterial solution (such as Escherichia coli ATCC 25922, with known concentration) for positive control testing to verify instrument sensitivity and response accuracy. Start the automatic cleaning program and rinse the flow system with 0.1% sodium hypochlorite or specialized cleaning solution for 10-15 minutes to kill residual microorganisms and prevent cross contamination. Rinse repeatedly with sterile water after rinsing until the effluent is neutral.
Step 3: Monthly Key Component Inspection (Engineering Maintenance)
Check the remaining amount of reagents: whether the fluorescent substrate, nutrient solution, buffer solution, etc. are sufficient, and replace them in advance if they are close to depletion;
Clean the optical window: Open the detection room and gently wipe the fluorescent sensor lens with a dust-free cloth dipped in anhydrous ethanol to remove protein deposits or scale;
Check the performance of the pump valve: confirm that the peristaltic pump speed is stable, the solenoid valve switch is smooth, and the pipeline is not aged or cracked;
Calibration turbidity compensation module (if integrated): Ensure accurate identification of biological signals in high turbidity water samples.
Step 4: Quarterly in-depth maintenance (professional service)
Replace vulnerable parts: including injection filter membrane, sealing ring, reaction cup or disposable detection chamber;
Check the cultivation temperature control system: verify whether the accuracy of the constant temperature module (usually 37 ℃ or 44.5 ℃) is within ± 0.5 ℃;
Calibrate the fluorescence intensity baseline and adjust the detection threshold;
Update system firmware and fix potential vulnerabilities;
Backup and clean the data storage module.