-
E-mail
luolinlin@jxzdkz.com
-
Phone
13439158498
-
Address
02, 1st to 3rd floors, Building 38, No. 2 Jingyuan North Street, Beijing Economic and Technological Development Zone (Beijing Pilot Free Trade Zone High end Industrial Zone Cluster)
Beijing Jingxiang Environmental Technology Co., Ltd
Briefly describe the common problems and corresponding solutions of Escherichia coli online analyzer
Date: 2025-10-16Read: 0
The Escherichia coli online analyzer achieves automated and continuous detection of the Escherichia coli population (including total Escherichia coli, heat-resistant Escherichia coli, and Escherichia coli) through immune analysis, enzyme-linked reaction, or biosensor technology, breaking through the time-consuming limitations of traditional cultivation methods and providing real-time data support for public health safety. In complex water samples and long-term operation, Escherichia coli online analyzers may experience false alarms, missed detections, and other issues due to reagent failure, sensor contamination, or interfering substances. Mastering the corresponding solutions to common problems is the key to ensuring the reliability of monitoring results.
Problem 1: High test results or continuous alarms (false positives)
Cause analysis: Reagent contamination, cross contamination, sensor residue, or presence of interfering substances (such as residual chlorine and heavy metals) in water.
Solution: Check if the reagent is expired or contaminated, and replace it with a new batch; Clean the sample flow path, reaction tank, and sensor; Install activated carbon filter or neutralizing agent to remove residual chlorine before injection; Regularly conduct blank testing with negative water samples to verify the background of the system.
Problem 2: Decreased detection sensitivity or inability to detect (false negative)
Cause analysis: reagent failure, decreased enzyme activity, light path contamination, or improper sample pretreatment.
Solution: Check the storage conditions of the reagents (such as refrigeration and avoiding light) to avoid repeated freezing and thawing; Check if the light source or photodetector is functioning properly; Clean optical components such as colorimetric dishes and fiber optic interfaces; Ensure that the sample filtration system is unobstructed to prevent particles from blocking the reaction.
Problem 3: The instrument cannot start or the control system is malfunctioning
Cause analysis: abnormal power supply, blown fuse, or software crash.
Solution: Check if the power supply voltage is stable and confirm that the grounding is good; Replace the fuse with the same specification; Restart the device or restore factory settings; Update the control software to a new version.
Question 4: Flow path blockage or liquid leakage
Cause analysis: The water sample contains too much suspended solids, the pipeline is aging, or the joints are loose.
Solution: Strengthen front-end preprocessing and install 0.45 μ m or 0.22 μ m filters; Regularly replace vulnerable parts such as peristaltic pump tubing and silicone tubing; Check the sealing rings of each joint, tighten or replace aging components.
Problem 5: Calibration failure or abnormal standard curve
Cause analysis: Failure of standard samples, inaccurate sample volume, or unstable reaction temperature.
Solution: Use freshly prepared standard bacterial solution or quality control product; Perform system cleaning procedures before calibration; Confirm that the constant temperature module is working properly and the reaction temperature is constant.