The online automatic monitoring instrument for Escherichia coli mainly uses the enzyme substrate method for detection. Its core principle is to use specific enzymes produced by E. coli metabolism to react with substrates and emit fluorescence or change color, and quantify the number of E. coli in water samples by detecting light signals or color changes. The following is a detailed introduction to the detection method:
1、 Detection principle
Enzyme substrate reaction: Specific enzymes (such as β - galactosidase) produced by E. coli metabolism react with substrates (such as fluorescent or chromogenic substrates) to produce fluorescent substances or change color.
Light signal detection: By detecting the fluorescence intensity or color change generated after the reaction, the number of Escherichia coli in the water sample can be quantified. The fluorescence intensity or color change is directly proportional to the number of Escherichia coli.
2、 Testing process
Sample collection and preprocessing:
Collect water samples through devices such as peristaltic pumps to ensure their representativeness and accuracy.
Perform necessary pre-treatment on the sample, such as filtration, dilution, etc., to meet the requirements of the detection instrument.
Enzyme substrate reaction:
Mix the preprocessed sample with the enzyme substrate reagent and initiate the reaction.
During the reaction process, enzymes produced by E. coli metabolism react with substrates to produce fluorescent substances or change color.
Optical signal detection and quantification:
Use photoelectric sensors or fluorescence detectors to detect the intensity or color changes of the light signal after the reaction.
Quantify the number of Escherichia coli in water samples based on the proportional relationship between the intensity or color change of the light signal and the quantity of E. coli.
Data output and warning:
Display the test results in real-time on the monitor screen and transmit them to the remote monitoring center through a data interface.
When the detection result exceeds the preset threshold, the monitor automatically sends a warning signal to remind relevant personnel to take measures.
3、 Testing advantages
Fast and accurate: able to provide test results within minutes, greatly reducing the time required for traditional laboratory testing.
High degree of automation: The entire process from sampling, processing to analysis has been basically automated, reducing manual intervention and improving efficiency.
Real time monitoring: capable of real-time data collection and transmission, timely detection of water quality abnormalities.
High sensitivity: capable of detecting low concentrations of Escherichia coli, suitable for various water quality monitoring scenarios.
4、 Application scenarios
Monitoring of drinking water source safety: Ensure that drinking water sources are not contaminated by Escherichia coli and safeguard public health.
Wastewater treatment plant effluent monitoring: Monitor the number of Escherichia coli in the effluent of the wastewater treatment plant to ensure that the effluent quality meets the standard.
Water quality monitoring in the food processing industry: monitoring the number of Escherichia coli in water used in the food processing industry to prevent food contamination.
Environmental water monitoring: Monitor the number of Escherichia coli in rivers, lakes, and other environmental water bodies to assess the degree of water pollution.