By optimizing the determination conditions of L-hydroxyproline in health products, an analytical method for quantitative detection of L-hydroxyproline in health products by liquid chromatography-mass spectrometry (LC-MS/MS) was established. The method used acid hydrolysis to treat L-hydroxyproline. The chromatographic column (EC-C18, 4.6 mm x 50 mm, 2.7 µ m) was used, and the mobile phase consisted of aqueous phase and acetonitrile eluted in a certain gradient at a flow rate of 0.5 mL/min. LC-MS/MS was used to detect L-hydroxyproline in positive ion mode, and external standard method was used for quantification. High performance liquid chromatography tandem mass spectrometry has high sensitivity, accuracy, and good reproducibility, and is suitable for the determination of L-hydroxyproline content in health products.

Recommended instruments:

Second hand Agilent 1260 liquid chromatograph

Second hand ABAPI 3200 Q-TRAPLiquid chromatography-mass spectrometry (LC-MS) instrument