A high-resolution rapid liquid chromatography-mass spectrometry (RRLC-MS/MS) method was established for the determination of Congo red in meat products. The homogenized sample was extracted using a mixture of acetonitrile and n-hexane solvent (1:1), and the acetonitrile extraction layer was centrifuged and directly tested on the machine. Using an Agilent XDB C18 chromatographic column, acetonitrile and 10 mmol/L ammonium formate aqueous solution were used as mobile phase gradient elution. Multiple reaction monitoring (MRM) negative ion mode detection was employed, with quantitative and qualitative ion pairs of m/z 325.0/416.0 and m/z 651.0/152.0, respectively. Quantification was performed using external standard method. The results showed that Congo Red had a good linear relationship within the range of 0.1-10 mg/L (r2>0.999), with a spiked recovery rate of 91% -102% and RSD of 1 2% -4. 0%, detection limit and quantification lower limit are 0.07 mg/kg and 0.18 mg/kg, respectively. This method is accurate, efficient, and can be used for rapid detection of Congo red dye in meat products.

Recommended instrument: 2Agilent liquid chromatography-mass spectrometry 1200-6410