Establish a liquid chromatography tandem mass spectrometry (LC-MS/MS) method for simultaneous detection of five organophosphate pesticide residues, including chlorpyrifos and chlorpyrifos, in cucumber. The sample was extracted with acetone, purified with Florisite, eluted with methanol water (the aqueous phase contains 0.1% formic acid) as the mobile phase gradient, separated with ZORBAX SB-C18 (5 µ m x 2.1 mm x 150mm) chromatographic column, ionized in the positive mode of electrospray ionization (ESI), and determined in the multi reaction monitoring mode (MRM). The results showed that the method standard curve had good linearity, with R2 ≥ 0.998; The limit of quantification for Chlorpyrifos and Chlorpyrifos is 0.01mg/kg, with a recovery rate of 72% -98% and a relative standard deviation (RSD) of ≤ 3.6%. Indicating that this method can achieve simultaneous and rapid determination of 5 types of organophosphorus pesticides, with high response values and low detection limits for the target pesticides, and can provide accurate and effective data support for risk assessment research in raw vegetables.

Recommended instruments:Second hand Agilent liquid chromatography-mass spectrometry instrument 1200-6410