Chemical pollution is widespread in biological and environmental ecosystems, and consuming fish products containing environmental hormones such as nonylphenol, octylphenol, and perfluorooctane sulfonate increases the likelihood of human exposure to these hormones.
Nonylphenol and octylphenol are extremely common chemical raw materials in detergents, textile products, and leather finishes. They belong to environmental hormones, which can interfere with endocrine and affect sexual development levels. They may lead to female precocious puberty, reduced male sperm count, and decreased quality. Nonylphenol is an important fine chemical raw material and intermediate. It appears as a colorless or light yellow liquid at room temperature, with a slight phenol odor. It is insoluble in water and soluble in acetone. It is mainly used in the production of surfactants, antioxidants, textile printing and dyeing auxiliaries, lubricant additives, pesticide emulsifiers, resin modifiers, resin and rubber stabilizers, and is an organic pollutant.

Bisphenol A, also known as BPA, is ubiquitous in the interior of mineral water bottles, medical devices, and food packaging. Every year, we produce 27 million tons of plastic containing BPA. But BPA can also cause endocrine disorders, threatening the health of fetuses and children. Obesity caused by cancer and metabolic disorders is also believed to be related to this. The European Union believes that baby bottles containing bisphenol A can induce precocious puberty, and from March 2, 2011, the production of baby bottles containing the chemical substance bisphenol A (BPA) has been banned.
Instrument preparation
LC600B binary high-pressure gradientliquid chromatographyThe system is equipped with one high-pressure gradient pump (including two constant current pumps and one gradient mixer), and the performance of the gradient mixer is *. The 16:9 TFT color screen supports offline input and display of all information through the instrument touch screen. Supports manual injection and can be equipped with an automatic sampler. It uses a syringe for quantitative analysis, the mobile phase passes through the needle, and the injection volume is controlled by a computer. The injection volume can be changed without replacing the quantitative ring, which greatly facilitates user operation.
Sample processing:
1. Take 1.25L~2L samples of mineral water and purified water, add 0.5 μ g of 4-n-NP internal standard solution, and adjust the pH to 2-3 with hydrochloric acid. Then extract the sample through SPE columns activated with 10mL of methanol and 10mL of deionized water (pH=2-3), respectively. Use a vacuum pump to remove the residual moisture from the SPE column, and then elute with 10mL of dichloromethane+methanol (volume ratio of 80:20) solution. The eluent was blown dry with nitrogen for 2 hours, then dissolved in methanol and brought to a volume of 1mL for analysis.
2. Take 100mL of carbonated beverage sample, add 0.5 μ g of 4-n-NP internal standard solution, and adjust the pH to 2-3 with hydrochloric acid. Then extract the sample through SPE columns activated with 10mL of methanol and 10mL of deionized water (pH=2-3), followed by rinsing with 6mL of methanol+water (volume ratio 40:60) solution. Use a vacuum pump to remove the residual water from column # 0, and then rinse with 10mL of dichloromethane+methanol (volume ratio 80:20) solution. Blow the eluent with nitrogen until almost dry, then dissolve it in methanol and make up to 1mL for analysis.
Chromatographic conditions:

Spectral analysis: