Chemiluminescence gel imaging system is a highly sensitive analytical instrument used in biological field. It is mainly used to detect and analyze chemiluminescence signals, and is widely used in life science, proteomics, molecular biology and other fields.
Chemiluminescence gel imaging system detects light signals generated by chemical reactions. In the experiment, antibodies or probes conjugated with enzymes (such as horseradish peroxidase, HRP) are added to the sample, and chemical substrates are added. When the substrate reacts with the enzyme, a chemiluminescence signal is generated, which is captured by a high-sensitivity sensor and converted into an image by the system. This feature enables it to detect weak light signals and is suitable for analysis of low abundance samples.
Performance Characteristics
High sensitivity: capable of detecting weak chemiluminescence signals with high sensitivity.
High resolution: it can provide a clear image, and the effective resolution is usually more than millions of pixels, which can accurately distinguish the strips in gel.
Wide dynamic range: It can detect both strong and weak signals simultaneously, ensuring image contrast and detail representation.
Multifunctionality: In addition to chemiluminescence imaging, it also supports multiple imaging modes such as fluorescence imaging and visible light imaging, suitable for different types of sample detection.
System Composition
Imaging darkbox: used to provide a dark environment to avoid interference from external light on imaging. Some imaging darkboxes also have good sealing and temperature control functions to ensure the stability of imaging.
Light source system: including excitation light source and transmission light source, etc. The excitation light source is used to excite fluorescent markers, and the transmission light source is used to observe the morphology of gel. Common light sources include ultraviolet lamps, LED lamps, etc.
Optical lenses: Large aperture fixed focus lenses, such as F0.95 lenses, are commonly used to improve light collection efficiency and enhance imaging sensitivity.
Detection camera: generally a research grade CCD or CMOS camera. CCD cameras have the characteristics of high sensitivity and low noise, while CMOS cameras have the advantages of high-speed imaging and low power consumption. The pixel count, resolution, cooling temperature, and other parameters of a camera have a significant impact on imaging quality.
Analysis software: used to control the operation of imaging systems, process and analyze collected images, such as automatically identifying bands, calculating molecular weight, analyzing optical density values, etc.