1、 The specific steps of peptide lysis technology process:

1. Selection of Cracking Reagents

It is crucial to choose the appropriate lysis reagent based on the peptide sequence and properties. Common cracking reagents include (TFA), (HCl), etc.

2. Cracking process

Mix peptides with lysis reagents, heat to a certain temperature, and maintain for a certain period of time to break the polypeptide chain. This step is the key to releasing the synthesized peptides on the solid-phase carrier.

3. Cooling

After the cracking is completed, cool the reaction solution to room temperature to stop the cracking reaction.

4. Centrifugal separation

Centrifuge the reaction solution to remove insoluble substances such as resin.

5. Solvent extraction

Extract the supernatant after centrifugation with an appropriate solvent to remove cracking reagents and other impurities.

　　2、 Peptide purification

Purification is an important step after lysis, aimed at removing impurities and separating the target peptide.

1. Purification method selection

According to the properties of the polypeptide, select appropriate purification methods, such as ion exchange chromatography, gel filtration, reverse phase chromatography, etc.

2. Purification process

Purify the peptide solution by passing it through a purification column.

3. Wash out

Wash the peptides on the purification column with an appropriate solvent.

4. Freeze drying

Freeze dry the purified peptide solution to obtain peptide powder.

The peptide lysis technology process is an important link in peptide production, and the technology and steps involved have a critical impact on ensuring the quality and efficiency of the final product. With the advancement of biotechnology and the growth of market demand, peptide lysis technology is constantly being optimized and innovated, providing a solid foundation for the development of the biopharmaceutical field.